Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ting-Yun Chiang
Date created: 2013-09-15 11:00:00
Date modified: 2015-05-08 01:09:09
AhpCp1000
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_K1104203_sequence (Version 1) |
Description
We improve the function of a BioBrick Part: AhpC promoter(K362001)designed by 2010 KIT-Tokyo team into four versions.On PartRegistry, the complex part(according to Ecocyc) composition contains hybrid promoters, shared TFBS (Transcription Factor Binding Site), and reverse promoter DsbG([[Figure. 6]]).In this part AhpC(mutated), we succesfully mutated the Pst1 site (ctgcag->ctacag) of K362001.Notes
AhpC promoter(K362001)has a Pst1 site in the sequence. We designed this part AhpC(mutated), and succesfully mutated the Pst1 site (ctgcag->ctacag) of K362001.Source
E. coli MG1655 genomic sequence| Sequence Annotation | Location | Component / Role(s) |
| (part of)DsbG coding start OxyR binding site1 OxyR binding site2 -35(AhpCp2) -10(AhpCp2) AhpCp2 ctgcag->ctacag g->a DsbGp OxyR binding site3 OxyR binding site4 -35(AhpCp1) -10(AhpCp1) AhpCp1 | 1,629 627,629 665,684 688,704 713,718 739,744 749,773 780,785 782,782 832,856 903,919 925,941 940,945 964,969 977,1000 | CDS feature/cds feature/start start_codon feature/binding non_covalent_binding_site feature/binding non_covalent_binding_site promoter feature/promoter promoter feature/promoter promoter feature/promoter feature/misc sequence_feature feature/mutation sequence_alteration feature/promoter promoter feature/binding non_covalent_binding_site feature/binding non_covalent_binding_site feature/promoter promoter promoter feature/promoter promoter feature/promoter |