Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ting-Yun Chiang
Date created: 2013-10-26 11:00:00
Date modified: 2015-05-08 01:09:10
OxyR device
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_K1104250_sequence (Version 1) |
Description
In order to enhance the strength and sensitivity of ROS-sensing promoters, we make E. coli continuously produce inactivated OxyR, so that ROS can affect promoters more easily by adding a device: Device1.Device1 in order to enhance the effect of ROS on E. coli is composed of :
#a constitutive promoter(Part:BBa_J23102)
#RBS(Part:BBa_B0034)
#activator OxyR(Part:BBa_K1104200)
#double terminator [http://parts.igem.org/Part:BBa_B0015 Part:BBa_B0015
Putting Device1 device:BBa_K1104250 upstream to promoter AhpCp2D1(Part:BBa_K1104204) remarkably enhanced the strength of promoter AhpCp2D1(Part:BBa_K1104204) as our experiment results shows.
Notes
.Source
OxyR sequence: http://biocyc.org/ECOLI/NEW-IMAGE?type=ENZYME&object=PD00214PartRegistry:
#a constitutive promoter(Part:BBa_J23102)
#RBS(Part:BBa_B0034)
#double terminator [http://parts.igem.org/Part:BBa_B0015 Part:BBa_B0015
| Access | Instance | Definition |
| public public public public | BBa_J23102 BBa_B0034 BBa_K1104200 BBa_B0015 | BBa_J23102 BBa_B0034 BBa_K1104200 BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23102 BBa_B0034 BBa_K1104200 BBa_B0015 | 1,35 44,55 62,979 988,1116 | BBa_J23102 BBa_B0034 BBa_K1104200 BBa_B0015 |