Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sandra Elisabeth Chaudron, Caroline Desmuget and Mareike Apelt
Date created: 2013-10-28 12:00:00
Date modified: 2015-05-08 01:09:10
INP_EYFP_Streptavidin Fusion Protein
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1111015_sequence (Version 1) |
Description
This part is a Fusion protein made respectively of an Ice Nucleation Protein (INP), EYFP and a Streptavidin protein.Notes
Obtained by gibson assembly using PCR products from BBa_K283010 and BBa_K523013 Biobricks.Source
To be used in E. Coli| Sequence Annotation | Location | Component / Role(s) |
| T7 promoter CAP binding site Lac LacZ INP Start BBa_K523013 INP BBa_K523013 INP-EYFP Linker BBa_K523013 EYFP PCR added EYFP-Strep Linker BBa_K283010 T7 Terminator EYFP start Streptavidin start Streptavidin stop Fusion Protein | 2,20 249,270 285,315 323,339 623,625 623,1555 1556,1606 1607,2323 2324,2341 2342,2728 2811,2838 1607,1609 2342,2344 2342,2344 623,2344 | feature/promoter promoter non_covalent_binding_site feature/binding promoter feature/promoter operator feature/operator start_codon feature/start feature/BioBrick engineered_region sequence_feature feature/misc engineered_region feature/BioBrick feature/misc sequence_feature feature/BioBrick engineered_region sequence_feature feature/misc feature/start start_codon start_codon feature/start stop_codon feature/stop feature/protein CDS |