Types | DnaRegion
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Roles | Generator
engineered_region
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Description
Includes the lacZ gene which encodes for the β-galactosidase attached with a linker (BBa_K105012) to the large subunit of the RuBisCo under the regulation of the pBad/araC promoter (BBa_I0500) and includes a RBS (BBa_B0034). The construct is for the delivery of the β-galactosidase and test reactions within the carboxysome.
Notes
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Source
The large subunits of the RuBisCo was obtained from the pHnCBS1D plasmid (plasmid provided by David Savage, Departments of Molecular & Cell Biology and Chemistry, University of California, Berkeley).