Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Belen Cespedes
Date created: 2013-09-17 11:00:00
Date modified: 2015-05-08 01:09:11
Targeting sequence for the delivery of the LacZ gene to the Carboxysome
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_K1113411_sequence (Version 1) |
Description
Includes the lacZ gene (BBa_I732005) which encodes for the β-galactosidase attached with a linker (BBa_K105012) to the large subunit of the RuBisCo (BBa_K1113701) under the regulation of the pBad/araC promoter (BBa_I0500) and includes a RBS (BBa_B0034). The construct is for the delivery of the β-galactosidase and test reactions within the carboxysome.Source of this part: The large subunits of the RuBisCo was obtained from the pHnCBS1D plasmid (plasmid provided by David Savage, Departments of Molecular & Cell Biology and Chemistry, University of California, Berkeley)
Notes
This part it constructed with a mutation in the Prefix (from ???cggccgc??? to ???atacctg???) which erase the NotI restriction site, this modification allow the correct design of primers for Gibson assembly. This part its also constructed on the pSB4K5 standard vectorSource
The large subunits of the RuBisCo was obtained from the pHnCBS1D plasmid (plasmid provided by David Savage, Departments of Molecular & Cell Biology and Chemistry, University of California, Berkeley)| Access | Instance | Definition |
| public public public public public | BBa_I0500 BBa_B0034 BBa_K1113701 BBa_K105012 BBa_I732005 | pBad/araC BBa_B0034 BBa_K1113701 BBa_K105012 LacZ |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_I0500 BBa_B0034 BBa_K1113701 BBa_K105012 BBa_I732005 | 1,1210 1219,1230 1237,2655 2664,2693 2700,5774 | pBad/araC BBa_B0034 BBa_K1113701 BBa_K105012 LacZ |