BBa_K1114019

BBa_K1114019 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1114019
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jake Awtry
Date created: 2013-09-06 11:00:00
Date modified: 2015-05-08 01:09:11

The MoClo format of BBa_J23116 with EB fusion sites.



Types
DnaRegion

Roles
Regulatory

promoter

Sequences BBa_K1114019_sequence (Version 1)

Description

Design Notes



This is the MoClo formatted part of BBa_J23116 with EB fusion sites. See the BBa_J23116 page for full information on the part.



This is a Level 0 MoClo part with flanking sites E on the 5' side and site B on the 3' side of the part.
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.


Summary of modifications from original part:


Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783056 .





Source




iGEM Distribution Kit


References



Notes

Design Notes



This is the MoClo formatted part of BBa_J23116 with EB fusion sites. See the BBa_J23116 page for full information on the part.



This is a Level 0 MoClo part with flanking sites E on the 5' side and site B on the 3' side of the part.
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.


Summary of modifications from original part:


Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783056 .





Source




iGEM Distribution Kit


References



Source

iGEM Distribution Kit

igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1114019/1