Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jake Awtry
Date created: 2013-09-06 11:00:00
Date modified: 2015-05-08 01:09:11
The MoClo format of BBa_R0063 with EB fusion sites.
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_K1114030_sequence (Version 1) |
Description
Design Notes
This is the MoClo formatted part of BBa_R0063 with AB fusion sites. See the BBa_R0063 page for full information on the part.
This is a Level 0 MoClo part with flanking sites E on the 5' side and site B on the 3' side of the part.
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.
Summary of modifications from original part:
Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783056 .
Source
iGEM Distribution Kit
References
Notes
Design Notes
This is the MoClo formatted part of BBa_R0063 with AB fusion sites. See the BBa_R0063 page for full information on the part.
This is a Level 0 MoClo part with flanking sites E on the 5' side and site B on the 3' side of the part.
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.
Summary of modifications from original part:
Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783056 .
Source
iGEM Distribution Kit