Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Traci Haddock
Date created: 2013-09-06 11:00:00
Date modified: 2015-05-08 01:09:11
MoClo format of a modified version of BBa_B0032 with BC fusion sites
| Types | DnaRegion |
| Roles | ribosome_entry_site RBS |
| Sequences | BBa_K1114102_sequence (Version 1) |
Description
This is the MoClo formatted part of a modified BBa_B0032 with BC fusion sites. See the BBa_B0032 page for full information on the part.
This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the sequence.
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.
This part is in a MoClo backbone that is a modified version of the pSB1C3 backbone. See BBA_K783058
Summary of modifications from original part:
Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites.
Notes
This is the MoClo formatted part of a modified BBa_B0032 with BC fusion sites. See the BBa_B0032 page for full information on the part.
This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the sequence.
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.
This part is in a MoClo backbone that is a modified version of the pSB1C3 backbone. See BBA_K783058
Summary of modifications from original part:
Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites.