BBa_K1114107

BBa_K1114107 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1114107
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Traci Haddock
Date created: 2013-09-06 11:00:00
Date modified: 2015-05-08 01:09:11

Bicistronic Design RBS 2, MoClo Format with BC fusion sites



    • Details

    Types
    DnaRegion

    Roles
    ribosome_entry_site

    RBS

    Sequences BBa_K1114107_sequence (Version 1)

    Description

    This is the MoClo formatted part of a bicistronic design ribosomal binding site 1 with BC fusion sites. This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the part.
    The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.


    Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055.




    Notes

    This is the MoClo formatted part of a bicistronic design ribosomal binding site 1 with BC fusion sites.
    This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the part.
    The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.


    Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055.




    Source

    This part was cloned off a BCD template from Sonya Iverson. Sonya Iverson based her designs for her BCD2 part off of a paper published by Mutalik, et al.

    Sequence Annotation Location Component / Role(s)
    BCD2
    MoClo Fusion Site B
    MoClo Fusion Site C
    		5,88
    1,4
    89,92
    		feature/rbs ribosome_entry_site
    sequence_feature feature/misc
    feature/misc sequence_feature

    igem#sampleStatus
    In stock
    igem#status
    Available
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K1114107/1
     

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