BBa_K1114107

BBa_K1114107 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1114107
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Traci Haddock
Date created: 2013-09-06 11:00:00
Date modified: 2015-05-08 01:09:11

Bicistronic Design RBS 2, MoClo Format with BC fusion sites



Types
DnaRegion

Roles
ribosome_entry_site

RBS

Sequences BBa_K1114107_sequence (Version 1)

Description

This is the MoClo formatted part of a bicistronic design ribosomal binding site 1 with BC fusion sites. This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the part.
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.


Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055.




Notes

This is the MoClo formatted part of a bicistronic design ribosomal binding site 1 with BC fusion sites.
This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the part.
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.


Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055.




Source

This part was cloned off a BCD template from Sonya Iverson. Sonya Iverson based her designs for her BCD2 part off of a paper published by Mutalik, et al.

Sequence Annotation Location Component / Role(s)
BCD2
MoClo Fusion Site B
MoClo Fusion Site C
5,88
1,4
89,92
feature/rbs ribosome_entry_site
sequence_feature feature/misc
feature/misc sequence_feature
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1114107/1