Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Traci Haddock
Date created: 2013-09-06 11:00:00
Date modified: 2015-05-08 01:09:11
Bicistronic design RBS 13, MoClo Format with BC fusion sites
| Types | DnaRegion |
| Roles | ribosome_entry_site RBS |
| Sequences | BBa_K1114110_sequence (Version 1) |
Description
This is the MoClo formatted part of a bicistronic design ribosomal binding site 13 with BC fusion sites.
This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the part.
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.
Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055.
Notes
This is the MoClo formatted part of a bicistronic design ribosomal binding site 13 with BC fusion sites.
This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the part.
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.
Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055.
Source
This part was cloned off a BCD template from Sonya Iverson. Sonya Iverson based her designs for her BCD2 part off of a paper published by Mutalik, et al.
| Sequence Annotation | Location | Component / Role(s) |
| MoClo Fusion Site B MoClo Fusion Site C BCD13 | 1,4 89,92 5,88 | sequence_feature feature/misc sequence_feature feature/misc feature/rbs ribosome_entry_site |