Types | DnaRegion
|
Roles | Terminator
terminator
|
Sequences | BBa_K1114302_sequence (Version 1)
|
Description
This is a Level 0 MoClo part with flanking sites D on the 5' side and site H on the 3' side of the part.
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.
Summary of modifications from original part:
Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See
BBa_.
Notes
This is a Level 0 MoClo part with flanking sites D on the 5' side and site H on the 3' side of the part.
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.
Summary of modifications from original part:
Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See
BBa_.
Source
iGEM kit