BBa_K1114421

BBa_K1114421 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1114421
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jake Awtry
Date created: 2013-09-06 11:00:00
Date modified: 2015-05-08 01:09:12

Level 1 MoClo Destination Vector with G and H fusion sites



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K1114421_sequence (Version 1)

Description

Design Notes



This is a MoClo destination vector containing the lacZ alpha fragment for blue-white screening with fusion sites G on the 5' side and site H on the 3' side of the part.
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA; Y =TGTG.

Summary of modifications from original part:
Backbone is a modified version of pSB1K3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_pSB1K3.


Source




iGEM Distribution Kit


References



Notes

Design Notes



This is a MoClo destination vector containing the lacZ alpha fragment for blue-white screening with fusion sites G on the 5' side and site H on the 3' side of the part.
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA; Y =TGTG.

Summary of modifications from original part:
Backbone is a modified version of pSB1K3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_pSB1K3.


Source




iGEM Distribution Kit


References



Source

iGEM Distribution Kit

igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1114421/1