Types | DnaRegion
|
Roles | Coding
CDS
|
Sequences | BBa_K1114422_sequence (Version 1)
|
Description
Design Notes
This is a MoClo destination vector containing the lacZ alpha fragment for blue-white screening with fusion sites A on the 5' side and site F on the 3' side of the part.
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA; Y =TGTG.
Summary of modifications from original part:
Backbone is a modified version of pSB1K3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See
BBa_pSB1K3.
Source
iGEM Distribution Kit
References
Notes
Design Notes
This is a MoClo destination vector containing the lacZ alpha fragment for blue-white screening with fusion sites A on the 5' side and site F on the 3' side of the part.
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA; Y =TGTG.
Summary of modifications from original part:
Backbone is a modified version of pSB1K3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See
BBa_pSB1K3.
Source
Source
iGEM Distribution Kit