MLS

BBa_K1119001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1119001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Wai In Hui

Date created: 2013-09-10 11:00:00

Date modified: 2015-05-08 01:09:13

Mitochondrial Leader Sequence with RFC25 standard

Details

• Types: DnaRegion
• Roles: polypeptide_domain; Protein_Domain
• Sequences: BBa_K1119001_sequence (Version 1)

Description

Mitochondrial leader sequence (MLS) helps direct protein to the mitochondria. When this sequence is cloned in frame at the N-terminus of the protein, it can target the recombinant protein to the mitochondria in mammalian cells. MLS will be removed upon translocation into the mitochondrial matrix. However, at least four additional amino acids (Ile-His-Ser-Leu) on this sequence are known to be retained on the N-terminus of the protein.

Since MLS always fuses with protein for function, this BioBrick is in RFC 25 standard, which facilitate protein fusion in order to introduce other protein into mitochondria.

Notes

No

Source

Invitrogen: pCMV/myc/mito (http://tools.lifetechnologies.com/content/sfs/manuals/pshooter_pcmv_man.pdf)

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Mitochondrial Leader Sequence	1,87	feature/protein, CDS

Other Properties

• igem#experience: Works
• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1119001/1

Member of these Collections

• //chassis/eukaryote/human
• //proteindomain/localization
• iGEM Parts Registry