xis

BBa_K112001 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K112001
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Bing Xia
Date created: 2008-10-19 11:00:00
Date modified: 2015-05-08 01:09:13

Xis from bacteriophage lambda, assembly standard 21



    • Details

    Types
    DnaRegion

    Roles
    Coding

    CDS

    Sequences BBa_K112001_sequence (Version 1)

    Description

    T4 holin gene without a start codon with a stop codon, in BBb format.

    This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:

    BBb Standard Description Page

    Source

    PCR bx3/bx2 on Bjh1301 (685 bp, EcoRI/BamHI/DpnI)
    Sub into pBca1256 (EcoRI/BamHI)
    Product is pBca1256-K112001

    bx3 Forward biobricking of { ccagtGAATTCatgAGATCTgcagcacctagaatatcattttcgccc
    bx2 Reverse biobricking of { cgttaGGATCCttatttagcccttcctaatattctggccgcc


    igem#experience
    None
     
    igem#sampleStatus
    It's complicated
    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K112001/1
     

    Attachments

    © 2018 Newcastle University, University of Utah, and collaborators
    About SynBioHub | View Source on Github | Report an Issue
    | v1.6.1 (86615526)