Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Bing Xia
Date created: 2008-09-02 11:00:00
Date modified: 2015-05-08 01:09:13
T4 holin, without start or stop codon, berkeley standard
| Types | DnaRegion |
| Roles | polypeptide_domain Protein_Domain |
| Sequences | BBa_K112003_sequence (Version 1) |
Description
T4 holin gene without start or stop codons, in BBb format.This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:
BBb Standard Description Page
Source
PCR bx3/bx4 on Bjh1301 (682 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112003
bx3 Forward Biobricking of {}
ccagtGAATTCatgAGATCTgcagcacctagaatatcattttcgccc
bx4 Reverse Biobricking of {}
cgttaGGATCCtttagcccttcctaatattctggcc