BBa_K112004

BBa_K112004 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K112004

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Bing Xia

Date created: 2008-09-02 11:00:00

Date modified: 2015-05-08 01:09:13

a~T4 holin! in BBb

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K112004_sequence (Version 1)

Description

T4 holin gene ready to have an rbs attached with a stop codon, in BBb format.

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This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:

BBb Standard Description Page

Notes

N/A

Source

PCR bx5/bx2 on Bjh1301		(692 bp, EcoRI/BamHI/DpnI)

Sub into pBca1256			(EcoRI/BamHI)

Product is pBca1256-K112004



bx5		Forward Biobricking of {a~T4 Holin!}

ccagtGAATTCatgAGATCTgtctatggcagcacctagaatatc

bx2		Reverse Biobricking of {a~T4 Holin!}

cgttaGGATCCttatttagcccttcctaatattctggccgcc

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K112004/1

Member of these Collections

• //cds/membrane/lysis
• //function/celldeath
• iGEM Parts Registry