BBa_K112005

BBa_K112005 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K112005

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Bing Xia

Date created: 2008-09-02 11:00:00

Date modified: 2015-05-08 01:09:13

rbs.T4 holin!

Details

• Types: DnaRegion
• Roles: engineered_region; Translational_Unit
• Sequences: BBa_K112005_sequence (Version 1)

Description

T4 holin gene with a native rbs and a stop codon, in BBb format.

---

This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:

BBb Standard Description Page

Notes

N/A

Source

PCR bx6/bx2 on Bjh1301		(704 bp, EcoRI/BamHI/DpnI)

Sub into pBca1256			(EcoRI/BamHI)

Product is pBca1256-K112005



bx6		Forward Biobricking of {rbs.T4 Holin!}

ccagtGAATTCatgAGATCTcttaaaaggagggtctatggcagcacctagaatatc

bx2		Reverse Biobricking of {rbs.T4 Holin!}

cgttaGGATCCttatttagcccttcctaatattctggccgcc

Other Properties

• igem#experience: None
• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K112005/1

Member of these Collections

• //function/celldeath
• iGEM Parts Registry