BBa_K112011

BBa_K112011 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K112011

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Bing Xia

Date created: 2008-09-02 11:00:00

Date modified: 2015-05-08 01:09:13

rbs.T4 antiholin! in BBb

Details

• Types: DnaRegion
• Roles: Translational_Unit; engineered_region
• Sequences: BBa_K112011_sequence (Version 1)

Description

T4 antiholin gene with a native rbs, with a stop codon, in BBb format.

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This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:

BBb Standard Description Page

Notes

N/A

Source

PCR bx12/bx8 on Bjh1303		(339 bp, EcoRI/BamHI/DpnI)

Sub into pBca1256			(EcoRI/BamHI)

Product is pBca1256-K112011



bx12	Forward biobricking of {rbs.T4 Antiholin!}

ccagtGAATTCatgAGATCTgataggaggcctttatggccttaaaagcaacagc

bx8		Reverse biobricking of {rbs.T4 Antiholin!}

cgttaGGATCCtcattcagtctccaatttaatgttc

Other Properties

• igem#experience: None
• igem#partStatus: Released HQ 2013
• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K112011/1

Member of these Collections

• //function/celldeath
• iGEM Parts Registry