BBa_K112014

BBa_K112014 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K112014

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Bing Xia

Date created: 2008-09-02 11:00:00

Date modified: 2015-05-08 01:09:13

T4 lysozyme> in BBb

Details

• Types: DnaRegion
• Roles: Protein_Domain; polypeptide_domain
• Sequences: BBa_K112014_sequence (Version 1)

Description

T4 lysozyme gene with a start codon, without a stop codon, in BBb format.

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This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:

BBb Standard Description Page

Notes

N/A

Source

PCR bx13/bx16 on Bjh1302		(523 bp, EcoRI/BamHI/DpnI)

Sub into pBca1256				(EcoRI/BamHI)

Product is pBca1256-K112014



bx13      Forward biobricking of {T4 Endolysin>}

ccagtGAATTCatgAGATCTatgaatatatttgaaatgttacg

bx16      Reverse biobricking of {T4 Endolysin>}

cgttaGGATCCtagatttttatacgcgtcccaagtgcc

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K112014/1

Member of these Collections

• //cds/enzyme/lysis
• //function/celldeath
• iGEM Parts Registry