BBa_K112100

BBa_K112100 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K112100
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Madhvi Venkatesh
Date created: 2008-08-13 11:00:00
Date modified: 2015-05-08 01:09:13

Cre enzyme without start codon



    • Details

    Types
    DnaRegion

    Roles
    polypeptide_domain

    Protein_Domain

    Sequences BBa_K112100_sequence (Version 1)

    Description

    Cre enzyme without start codon

    Notes

    This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites.

    PCR mv001/mv002 on pBca9145-Bca1010 (1060bp, EcoRI/BamHI/DpnI)
    Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
    Product is pBca1256-K112100

    mv001 Forward Biobricking of ccataGAATTCatgAGATCTtccaatttactgaccgtacacc
    mv002 Reverse Biobricking of cgttaGGATCCttaatcgccatcttccagc

    Source

    PCR mv001/mv002 on pBca9145-Bca1010 (1060bp, EcoRI/BamHI)
    Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
    Product is pBca1256-K112100

    igem#experience
    None
     
    igem#sampleStatus
    It's complicated
    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K112100/1
     

    Attachments

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