BBa_K112101

BBa_K112101 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K112101
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Madhvi Venkatesh
Date created: 2008-10-20 11:00:00
Date modified: 2015-05-08 01:09:13

Cre enzyme without stop codon



    • Details

    Types
    DnaRegion

    Roles
    Protein_Domain

    polypeptide_domain

    Sequences BBa_K112101_sequence (Version 1)

    Description

    Cre enzyme without stop codon

    Notes

    This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites.


    
PCR mv003/mv004 on pBca9145-Bca1010    (1060bp, EcoRI/BamHI/DpnI)
    
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
    
Product is pBca1256-K112101
    


    
mv003	Forward Biobricking of Cre>
    
ccataGAATTCatgAGATCTatgtccaatttactgaccgtac
    
mv004	Reverse Biobricking of Cre>
    
cgttaGGATCCatcgccatcttccagcaggcg

    Source


    
PCR mv003/mv004 on pBca9145-Bca1010    (1060bp, EcoRI/BamHI/DpnI)
    
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
    
Product is pBca1256-K112101


    igem#experience
    None
     
    igem#sampleStatus
    It's complicated
    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K112101/1
     

    Attachments

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