BBa_K112104

BBa_K112104 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K112104

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Madhvi Venkatesh

Date created: 2008-10-20 11:00:00

Date modified: 2015-05-08 01:09:14

BamHI endonuclease without stop codon

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K112104_sequence (Version 1)

Description

BamHI endonuclease without stop codon

Notes

This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites.

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PCR mv005/mv006 on pBca9145-I716210    (673bp, EcoRI/BamHI/DpnI)

Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)

Product is pBca1256-K112103



mv005	Forward Biobricking of r.BamH1!

ccataGAATTCatgAGATCTatggaagtagaaaaagagtttattactgatgaagc

mv006	Reverse Biobricking of r.BamH1!

cgttaGGATCCctatttgttttcaactttatctttcc

Source

PCR mv005/mv006 on pBca9145-I716210    (673bp, EcoRI/BamHI/DpnI)

Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)

Product is pBca1256-K112103

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K112104/1

Member of these Collections

• iGEM Parts Registry