BBa_K112106

BBa_K112106 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K112106

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Madhvi Venkatesh

Date created: 2008-10-20 11:00:00

Date modified: 2015-05-08 01:09:14

BglII endonuclease

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K112106_sequence (Version 1)

Description

BglII endonuclease with start and stop codons

Notes

This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites.

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PCR mv009/mv010 on pBca9145-Bca1147    (703bp, EcoRI/BamHI/DpnI)

Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)

Product is pBca1256-K112106



mv009	Forward Biobricking of r.BglII!

ccataGAATTCatgAGATCTatgaagattgatataacggactataaccatgc

mv010	Reverse Biobricking of r.BglII!

cgttaGGATCCttaatatgtcacgattgttcctc

Source

PCR mv009/mv010 on pBca9145-Bca1147    (703bp, EcoRI/BamHI/DpnI)

Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)

Product is pBca1256-K112106

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• rdf-schema#seeAlso: Q45488
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K112106/1

Member of these Collections

• //cds/enzyme/endonuclease/restriction
• iGEM Parts Registry