BBa_K112107

BBa_K112107 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K112107

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Madhvi Venkatesh

Date created: 2008-10-20 11:00:00

Date modified: 2015-05-08 01:09:14

BglII endonuclease without stop codon

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K112107_sequence (Version 1)

Description

BglII endonuclease without stop codon

Notes

This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites.

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PCR mv009/mv011 on pBca9145-Bca1147    (700bp, EcoRI/BamHI/DpnI)

Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)

Product is pBca1256-K112107



mv009	Forward Biobricking of r.BglII!

ccataGAATTCatgAGATCTatgaagattgatataacggactataaccatgc

mv011	Reverse Biobricking of r.BglII>

cgttaGGATCCatatgtcacgattgttcctc

Source

PCR mv009/mv011 on pBca9145-Bca1147    (700bp, EcoRI/BamHI/DpnI)

Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)

Product is pBca1256-K112107

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K112107/1

Member of these Collections

• //cds/enzyme/endonuclease/restriction
• iGEM Parts Registry