BBa_K112108

BBa_K112108 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K112108

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Madhvi Venkatesh

Date created: 2008-10-20 11:00:00

Date modified: 2015-05-08 01:09:14

BglII endonuclease without start or stop codons

Details

• Types: DnaRegion
• Roles: Protein_Domain; polypeptide_domain
• Sequences: BBa_K112108_sequence (Version 1)

Description

BglII endonuclease without start or stop codons

Notes

This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites.

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PCR mv012/mv011 on pBca9145-Bca1147    (697bp, EcoRI/BamHI/DpnI)

Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)

Product is pBca1256-K112108



mv012	Forward Biobricking of 

ccataGAATTCatgAGATCTaagattgatataacggactataacc

mv011	Reverse Biobricking of r.BglII>

cgttaGGATCCatatgtcacgattgttcctc

Source

PCR mv012/mv011 on pBca9145-Bca1147    (697bp, EcoRI/BamHI/DpnI)

Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)

Product is pBca1256-K112108

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K112108/1

Member of these Collections

• //cds/enzyme/endonuclease/restriction
• iGEM Parts Registry