BBa_K112112

BBa_K112112 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K112112

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Bing Xia

Date created: 2008-10-20 11:00:00

Date modified: 2015-05-08 01:09:14

Ligase with a start codon without a stop codon

Details

• Types: DnaRegion
• Roles: polypeptide_domain; Protein_Domain
• Sequences: BBa_K112112_sequence (Version 1)

Description

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This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:

BBb Standard Description Page

Source

PCR mv021/mv023 on pBca1256-K112109    (1492bp, EcoRI/BamHI/DpnI)

Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)

Product is pBca1256-K112112



mv021	Forward Biobricking of ligase!

ccataGAATTCatgAGATCTatgattcttaaaattctgaacg

mv023	Reverse Biobricking of ligase>

cgttaGGATCCtagaccagttacctcatg

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K112112/1

Member of these Collections

• //cds/enzyme/ligase
• iGEM Parts Registry