BBa_K112113

BBa_K112113 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K112113
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Bing Xia
Date created: 2008-10-20 11:00:00
Date modified: 2015-05-08 01:09:14

Ligase without start or stop codons



    • Details

    Types
    DnaRegion

    Roles
    Protein_Domain

    polypeptide_domain

    Sequences BBa_K112113_sequence (Version 1)

    Description


    This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:

    BBb Standard Description Page

    Source


    
PCR mv022/mv023 on pBca1256-K112109    (1489bp, EcoRI/BamHI/DpnI)
    
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
    
Product is pBca1256-K112113
    


    
mv022	Forward Biobricking of 
ccataGAATTCatgAGATCTattcttaaaattctgaacg
    
mv023	Reverse Biobricking of ligase>
    
cgttaGGATCCtagaccagttacctcatg


    igem#experience
    None
     
    igem#sampleStatus
    It's complicated
    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K112113/1
     

    Attachments

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