BBa_K112114

BBa_K112114 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K112114
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Bing Xia
Date created: 2008-10-20 11:00:00
Date modified: 2015-05-08 01:09:14

a~Ligase!



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K112114_sequence (Version 1)

Description



This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:

BBb Standard Description Page

Source


PCR mv024/mv014 on pBca1256-K112109 (1499bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112114


mv024 Forward Biobricking of a~ligase!
ccataGAATTCatgAGATCTaacaatgattcttaaaattctgaacg
mv014 Reverse Biobricking of ligase!
cgttaGGATCCtcatagaccagttacctcatg


igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K112114/1