BBa_K112115

BBa_K112115 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K112115

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Bing Xia

Date created: 2008-10-20 11:00:00

Date modified: 2015-05-08 01:09:14

a~Cre

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K112115_sequence (Version 1)

Description

Cre recombinase gene, with a spacer for the rbs prepended.

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This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:

BBb Standard Description Page

Source

PCR mv025/mv002 on pBca9145-Bca1010    (1067bp, EcoRI/BamHI/DpnI)

Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)

Product is pBca1256-K112115



mv025	Forward Biobricking of a~Cre!

ccataGAATTCatgAGATCTttaaatgtccaatttactgaccgtacacc

mv002	Reverse Biobricking of 
cgttaGGATCCttaatcgccatcttccagc

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K112115/1

Member of these Collections

• //function/recombination
• //function/recombination/cre
• iGEM Parts Registry