BBa_K112233

BBa_K112233 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K112233

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Bing Xia

Date created: 2008-10-20 11:00:00

Date modified: 2015-05-08 01:09:15

< phoA >

Details

• Types: DnaRegion
• Roles: Protein_Domain; polypeptide_domain
• Sequences: BBa_K112233_sequence (Version 1)

Description

The phoA enzyme without start or stop codons.

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This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:

BBb Standard Description Page

Source

Construction of {} Biobrick Part K112233

PCR mea036/mea037 on pBca9145-Bca1126		(1219 bp, EcoRI/BamHI)

Sub into pBjh1601AC-Bca1144			(EcoRI/BamHI, 3195,910 L)

Product is pBjh1601AC-K112233



mea036   Forward Biobricking of {}	cgatagaattcatgAGATCTGGAGATTCTG

mea037   Reverse Biobricking of {}	cgttaGGATCCCTTCAGGCCCAGCGCCGCTTTCATG

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K112233/1

Member of these Collections

• //cds/enzyme
• iGEM Parts Registry