xis/int

BBa_K112234 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K112234

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Molly Allen

Date created: 2008-10-20 11:00:00

Date modified: 2015-05-08 01:09:15

{rbs.xis.int!} The bacteriophage lambda xis/integrase gene with native rbs and stop codon
assembly

Details

• Types: DnaRegion
• Roles: engineered_region; Translational_Unit
• Sequences: BBa_K112234_sequence (Version 1)

Description

This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:

BBb Standard Description Page

Notes

The xis and int genes naturally overlap, so instead of trying to isolate both the xis and int and put them together, I decided to make them one entire basic part.

Source

pAH57 plasmid

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
rbs	1,16	ribosome_entry_site, feature/rbs
xis	20,237	CDS, feature/cds
int	216,1286	feature/cds, CDS
start	20,22	feature/start, start_codon
stop	1284,1286	feature/stop, stop_codon

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K112234/1

Member of these Collections

• //function/recombination/lambda
• iGEM Parts Registry