BBa_K1122679

BBa_K1122679 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1122679

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Aleksandra Lewicka, Jan Lyczakowski

Date created: 2013-08-29 11:00:00

Date modified: 2015-05-08 01:09:15

pSpac - LacZ - fused pdc and adhB

Details

• Types: DnaRegion
• Roles: Translational_Unit; engineered_region
• Sequences: BBa_K1122679_sequence (Version 1)

Description

This translational unit enables expression of pyruvate decarboxylase (pdc) and alcohol dehydrogenase B (adhB) fused peptides in Bacillus subtilis using xylose induction. Production of pdc and adhB fused enzymes allows for conversion of pyruvate to ethanol via an acetaldehyde intermediate. The fusion state generates increased ethanol yeilds in aerobic conditions what should have a beneficial effect on EtOH production in B. subtilis

Notes

Part was generated from EdiBrick - fused pdc and adhB (K1122674) via MABEL utilising standardised primers.

Presence of LacZ alpha peptide enables blue - white screening in E. coli when the part is inserted into an E. coli - B. subtilis shuttle vector.

Source

synthetic

Components

Access	Instance	Definition
public	BBa_K1122677	BBa_K1122677
public	BBa_K1122673	pET fusion

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K1122677	1,390	BBa_K1122677
BBa_K1122673	399,3412	pET fusion

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1122679/1

Member of these Collections

• iGEM Parts Registry