| Types | DnaRegion
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| Roles | CDS
Coding
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| Sequences | BBa_K1123021_sequence (Version 1)
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Description
This part contains the DNA sequence of a protein of our own design. We first repeated the Lysine-Serine amino acid pair 6 times to obtain a sequence of 12 amino acids. This sequence was then repeated a total of 36 times. Between these repeats no extra amino acids were added. The idea behind this protein was to provide ourselves with a high concentration of amino acids with amide groups which we could then use to provide CEST contrast in an MRI. The DNA sequence can be placed behind any compatible promoter and the protein will be expressed.
Notes
We had designed the entire protein sequence from scratch, repeating the Lysine-Serine amino acid pair a total number of 216 times. We then randomised the codon usage for optimization in E.coli.
Source
This protein is one of our own design. Created for use in an MRI to provide CEST contrast. Whilst we designed the sequence ourselves the idea for using Poly amide amino acids came from the following article: "New ???Multicolor??? Polypeptide Diamagnetic Chemical Exchange Saturation Transfer (DIACEST) Contrast Agents for MRI" by McMahon et al. Here they outline the use of Poly amide amino acids chains, although the chains used here have a length of 12 amino acids. To increase the amide amino acid concentration we therefore decided to increase this to 432.
