BBa_K112307

BBa_K112307 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K112307

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Aron Lau

Date created: 2008-10-20 11:00:00

Date modified: 2015-05-08 01:09:16

{< lambda holin!} in BBb format

Details

• Types: DnaRegion
• Roles: Protein_Domain; polypeptide_domain
• Sequences: BBa_K112307_sequence (Version 1)

Description

lambda holin with stop codon and no start codon in BBb format
More information about the BBb Format is available at:

BBb Standard Description Page

Notes

PCR al0009/al0008 on pSB3C6-Bjh1502     (346bp, EcoRI/BamHI/DpnI)

Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)

Product is pBca1256-K112307



al0009	Forward Biobricking of lambda holin with stop codon and no start codon

CCATAGAATTCatgAGATCTccagaaaaacatgacctgttggcc

al0008	Reverse Biobricking of lambda holin with stop codon and no start codon

CGttaGGATCCttattgatttctaccatcttctactccggc

Source

pSB3C6-Bjh1502

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K112307/1

Member of these Collections

• //cds/membrane/lysis
• //function/celldeath
• iGEM Parts Registry