BBa_K112310

BBa_K112310 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K112310

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Aron Lau

Date created: 2008-10-20 11:00:00

Date modified: 2015-05-08 01:09:16

{a~lambda holin!}
adheres to Berkeley standard

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K112310_sequence (Version 1)

Description

lambda holin ready for rbs addition and has stop codon in BBb format.
More information about the BBb Format is available at:

BBb Standard Description Page

Notes

PCR al0011/al0008 on pSB3C6-Bjh1502     (353bp, EcoRI/BamHI/DpnI)

Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)

Product is pBca1256-K112310



al0011	Forward Biobricking of lambda holin with spacer

CCATAGAATTCatgAGATCTgaagatgccagaaaaacatgacctg

al0008	Reverse Biobricking of lambda holin with spacer

CGttaGGATCCttattgatttctaccatcttctactccggc

Source

pSB3C6-Bjh1502

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K112310/1

Member of these Collections

• //cds/membrane/lysis
• //function/celldeath
• iGEM Parts Registry