BBa_K112311

BBa_K112311 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K112311
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Aron Lau
Date created: 2008-10-20 11:00:00
Date modified: 2015-05-08 01:09:16

{rbs.lambda holin!} in BBb format



Types
DnaRegion

Roles
Translational_Unit

engineered_region

Sequences BBa_K112311_sequence (Version 1)

Description

lambda holin with rbs site and stop codon in BBb format.
More information about the BBb Format is available at:

BBb Standard Description Page

Notes


PCR al0012/al0008 on pSB3C6-Bjh1502 (369bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112311


al0012 Forward Biobricking of lambda holin with rbs site
CCATAGAATTCatgAGATCTattgggggtaagacCtgaag
al0008 Reverse Biobricking of lambda holin with rbs site
CGttaGGATCCttattgatttctaccatcttctactccggc


Source

pSB3C6-Bjh1502

igem#experience
Works
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K112311/1