BBa_K112313

BBa_K112313 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K112313

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Aron Lau

Date created: 2008-10-20 11:00:00

Date modified: 2015-05-08 01:09:16

{< lambda antiholin!}
adheres to Berkeley standard

Details

• Types: DnaRegion
• Roles: polypeptide_domain; Protein_Domain
• Sequences: BBa_K112313_sequence (Version 1)

Description

lambda antiholin with stop codon and no start codon. This part is actually in BBb format.

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This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:

BBb Standard Description Page

Notes

PCR al0014/al0008 on pSB3C6-Bjh1503     (352bp, EcoRI/BamHI/DpnI)

Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)

Product is pBca1256-K112313



al0014	Forward Biobricking of lambda antiholin with stop codon and no start codon

CCATAGAATTCatgAGATCTaagCtgccagaaaaacatgacctg

al0008	Reverse Biobricking of lambda antiholin with stop codon and no start codon

CGttaGGATCCttattgatttctaccatcttctactccggc

Source

pSB3C6-Bjh1503

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K112313/1

Member of these Collections

• //cds/membrane/lysis
• //function/celldeath
• iGEM Parts Registry