BBa_K112314

BBa_K112314 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K112314

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Aron Lau

Date created: 2008-10-20 11:00:00

Date modified: 2015-05-08 01:09:16

{ lambda antiholin >}
adheres to Berkeley standard

Details

• Types: DnaRegion
• Roles: Protein_Domain; polypeptide_domain
• Sequences: BBa_K112314_sequence (Version 1)

Description

lambda antiholin with start codon and no stop codon. This part is actually in BBb format.

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This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:

BBb Standard Description Page

Notes

PCR al0013/al0010 on pSB3C6-Bjh1503     (352bp, EcoRI/BamHI/DpnI)

Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)

Product is pBca1256-K112314



al0013	Forward Biobricking of lambda antiholin with start codon and no stop codon

CCATAGAATTCatgAGATCTatgaagCtgccagaaaaacatgacc

al0010	Reverse Biobricking of lambda antiholin with start codon and no stop codon

CGttaGGATCCttgatttctaccatcttctactccggc

Source

pSB3C6-Bjh1503

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K112314/1

Member of these Collections

• //cds/membrane/lysis
• //function/celldeath
• iGEM Parts Registry