BBa_K112317

BBa_K112317 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K112317

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Aron Lau

Date created: 2008-10-20 11:00:00

Date modified: 2015-05-08 01:09:16

{rbs.lambda antiholin!} in BBb format

Details

• Types: DnaRegion
• Roles: engineered_region; Translational_Unit
• Sequences: BBa_K112317_sequence (Version 1)

Description

lambda antiholin with native rbs site and stop codon. This part is actually in BBb format.

---

This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:

BBb Standard Description Page

Notes

PCR al0016/al0008 on pSB3C6-Bjh1503     (375bp, EcoRI/BamHI/DpnI)

Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)

Product is pBca1256-K112317



al0016	Forward Biobricking of lambda antiholin with rbs site

CCATAGAATTCatgAGATCTccccttattgggggtaagacatg

al0008	Reverse Biobricking of lambda antiholin with rbs site

CGttaGGATCCttattgatttctaccatcttctactccggc

Source

pSB3C6-Bjh1503

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K112317/1

Member of these Collections

• //function/celldeath
• iGEM Parts Registry