BBa_K112321

BBa_K112321 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K112321

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Aron Lau

Date created: 2008-10-21 11:00:00

Date modified: 2015-05-08 01:09:16

{H-NS!} using MG1655 reverse oligo in BBb format

Details

• Types: DnaRegion
• Roles: Regulatory; promoter
• Sequences: BBa_K112321_sequence (Version 1)

Description

{H-NS!} cloned using non complementary primer in BBb format. This part is actually in BBb format.

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This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:

BBb Standard Description Page

Notes

PCR JH088F/JHO88R on Salmonella L2.     (435bp, EcoRI/BamHI/DpnI)

Sub into pBjh1601AC                      (EcoRI/BamHI, 3195+910, L)

Product is pBjh1601AC-K112321



JH088F   Forward biobricking of {H-NS!}   

cgataGAATTCatgAGATCTATGAGCGAAGCACTTAAAATTC

JH088R   Reverse biobricking of {H-NS!}   

cgttaGGATCCTTATTGCTTGATCAGGAAATCG

Source

Salmonella L2

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K112321/1

Member of these Collections

• iGEM Parts Registry