BBa_K112612

BBa_K112612 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K112612

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Sherine Cheung

Date created: 2008-10-21 11:00:00

Date modified: 2015-05-08 01:09:18

a~PhoA pp> in BBb

Details

• Types: DnaRegion
• Roles: polypeptide_domain; Protein_Domain
• Sequences: BBa_K112612_sequence (Version 1)

Description

This is a phoA prepro with an N4 spacer, an atg start codon, but no stop codon.

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This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:


BBb Standard Description Page

Notes

N/A

Source

Construction of {a~PhoA>} basic part K112612 

         

PCR sc012/sc013 on MG1655                     (98 bp, EcoRI/BamHI)                              

Sub into pBca1256                             (EcoRI/BamHI, 2472+910, L)

Product is pBca1256-K112605



sc012      Fwd biobricking of {a~prepro>}

cgataGAATTCatgAGATCTtaaaAtgaaacaaagcactattgcactggc

sc013    Reverse biobricking of {prepro>}

ccagtGGATCCggcttttgtcacaggggtaaac

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K112612/1

Member of these Collections

• iGEM Parts Registry