BBa_K112614

BBa_K112614 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K112614

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Sherine Cheung

Date created: 2008-10-21 11:00:00

Date modified: 2015-05-08 01:09:18

rbs.PhoA pp> in BBb

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K112614_sequence (Version 1)

Description

This is a phoA prepro with a native rbs and start codon, but without a stop codon. It is in BBb format.

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This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:


BBb Standard Description Page

Notes

N/A

Source

Construction of {rbs_PhoA>} basic part K112614

         

PCR sc010/sc013 on MG1655                     (112 bp, EcoRI/BamHI)                              

Sub into pBca1256                             (EcoRI/BamHI, 2472+910, L)

Product is pBca1256-K112605



sc010      Fwd biobricking of {rbs_prepro>}

cgataGAATTCatgAGATCTgtacatggagaaaataaaAtgaaacaaagcac

sc013    Reverse biobricking of {prepro>}

ccagtGGATCCggcttttgtcacaggggtaaac  

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K112614/1

Member of these Collections

• iGEM Parts Registry