S tag

BBa_K112704 Version 1

Component

This part has been discontinued.

Source: http://parts.igem.org/Part:BBa_K112704

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Bing Xia

Date created: 2008-10-20 11:00:00

Date modified: 2015-05-08 01:09:18

Details

• Types: DnaRegion
• Roles: tag; Tag
• Sequences: BBa_K112704_sequence (Version 1)

Description

This S-tag can be added after a protein part.

---

This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:

BBb Standard Description Page

Source

Construction of 


Wobble PCR of dv011/dv012		(60 bp, EcoRI/BamHI/DpnI)

Sub into pBca1256			(EcoRI/BamHI)

Product is pBca1256-K112704



dv011		Forward Biobricking of 
cgataGAATTCatgAGATCTaaagaaaccgctgctgctaaattcgaacgcc



dv012		Reverse Biobricking of 
cgttaGGATCCttagctgtccatgtgctggcgttcgaatttagcagc

Other Properties

• igem#experience: None
• igem#sampleStatus: Discontinued
• igem#status: Deleted
• synbiohub#ownedBy: user/myers
• synbiohub#ownedBy: user/james
• synbiohub#topLevel: BBa_K112704/1

Member of these Collections

• iGEM Parts Registry