BBa_K112706

BBa_K112706 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K112706
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Bing Xia
Date created: 2008-10-20 11:00:00
Date modified: 2015-05-08 01:09:18

Pspv2 from Salmonella



    • Details

    Types
    DnaRegion

    Roles
    Regulatory

    promoter

    Sequences BBa_K112706_sequence (Version 1)

    Description

    The spv2 promoter.

    This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:

    BBb Standard Description Page

    Source


    
Construction of  basic part K112706
    

    
PCR of dv015/dv016 on pBca1037          (500bp, EcoRI/BamHI/DpnI)
    
Sub into pBca1256                       (EcoRI/BamHI, 2472+ 500, L)
    
Product is pBca1256-K112706
    


    
dv015 Forward biobricking of 
    
cgataGAATTCatgAGATCTccttatgcagcgtaagggccgcaac
    

    
dv016 Reverse biobricking of 
    
cgttaGGATCCGATAATGTtTGCAGGGGAATTATTTTG


    igem#experience
    None
     
    igem#sampleStatus
    Not in stock
    igem#status
    Unavailable
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K112706/1
     

    Attachments

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