BBa_K112709

BBa_K112709 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K112709

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Bing Xia

Date created: 2008-10-20 11:00:00

Date modified: 2015-05-08 01:09:18

b1006

Details

• Types: DnaRegion
• Roles: Terminator; terminator
• Sequences: BBa_K112709_sequence (Version 1)

Description

The b1006 terminator.

---

This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:

BBb Standard Description Page

Source

Construction of  basic part K112709

 

Wobble PCR of dv021/dv022              (66bp, EcoRI/BamHI/DpnI)

Sub into pBca1256                      (EcoRI/BamHI, 2472+66, L)

Product is pBca1256-K112709



dv021 Forward biobricking of b1006

cgataGAATTCatgAGATCTaaaaaaaaaccccgcccctgacagggcgg



dv022 Reverse biobricking of b1006

GGATCCtaaaaaaaaccccgccctgtcaggggcggggtttttt

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K112709/1

Member of these Collections

• iGEM Parts Registry