Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Daniel Cabrera
Date created: 2013-08-28 11:00:00
Date modified: 2015-05-08 01:09:19
Targetted Methylation Assay
| Types | DnaRegion |
| Roles | Plasmid_Backbone plasmid_vector |
| Sequences | BBa_K1128001_sequence (Version 1) |
Description
This part allows a user to quickly and easily screen site specific methylating proteins for activity and specificity.Notes
We worked to make the assay clear and as friendly to work with biobricks as possible.Source
Modified bacterial expression plasmid.| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1128001 BBa_R0010 BBa_J04450 CAP -35 -10 LacI start BBa_B0034 conserved BBa_E1010 mrfp1 barcode BBa_B0010 stem_loop BBa_B0012 T7 TE polya stop | 1,6650 6659,6858 6659,7727 6747,6784 6795,6800 6819,6824 6824,6858 6831,6831 6867,6878 6871,6874 6885,7565 6885,7559 7566,7590 7599,7678 7610,7653 7687,7727 7694,7713 7714,7727 7720,7720 | engineered_region feature/BioBrick engineered_region feature/BioBrick feature/BioBrick engineered_region feature/binding non_covalent_binding_site feature/promoter promoter feature/promoter promoter feature/binding non_covalent_binding_site feature/start start_codon engineered_region feature/BioBrick feature/conserved conserved_region engineered_region feature/BioBrick CDS feature/cds feature/barcode engineered_tag feature/BioBrick engineered_region feature/stem_loop stem_loop engineered_region feature/BioBrick stem_loop feature/stem_loop polyA_site feature/polya stop_codon feature/stop |