| Types | DnaRegion
|
| Roles | polypeptide_domain
Protein_Domain
|
| Sequences | BBa_K1128007_sequence (Version 1)
|
Description
Methyl CpG binding protein 2 (MECP2) is a mammalian protein that binds to methylated CpG sites by recruiting histone deacetylases, and is essential for embryonic development. The Penn iGEM team is using this protein to test the feasibility of transcriptional silencing using CpG methylation in bacteria using a cell-free assay and a methylated red fluorescent protein gene. The possibility of transcriptional silencing in E. Coli using methylation that is completely orthogonal to native E. Coli methylation can open up whole new for synthetic biologists, giving them a layer of control on genetic systems that has yet to be touched by traditional synthetic biology. This part can be purified for use in cell-free assays, and used for CpG methylated transcriptional silencing studies.
Notes
We are currently in the process of optimizing this part for use in biobrick cloning by making it compatible with biobrick assemble standards, and will submit to the registry when it is complete.
Source
This protein comes from the Mus Musculus genome, optimized for expression in bacteria.
