Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: UBC iGEM 2013
Date created: 2013-08-29 11:00:00
Date modified: 2015-05-08 01:09:19
CaXMT1 under pTET constitutive promoter
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1129016_sequence (Version 1) |
Description
CaXMT1 is the gene coding for the first enzyme of the caffeine biosynthesis pathway, known as xanthosine methyltransferase, when starting with xanthosine as substrate. TU Munich's 2012 team isolated this gene and added a strep tag at the end, in addition to transferring it all into yeast. We modified it further by removing the yeast consensus sequence and placing the gene and strep tag behind a constitutive promoter and bacterial consensus sequence. This system was moved into a prokaryote, specifically E. coli strain K12.Notes
We have yet to test for expression, and we may find that codon optimization is needed.Source
Combined two parts from this year's and the 2012 Distribution Kit. We used the constitutive promoter BBa_J23118 and TU Munich's BioBrick BBa_K801070.| Access | Instance | Definition |
| public public public public | BBa_J23118 BBa_B0034 BBa_K801070 BBa_B0015 | BBa_J23118 BBa_B0034 BBa_K801070 BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23118 BBa_B0034 BBa_K801070 BBa_B0015 | 1,35 44,55 64,1221 1230,1358 | BBa_J23118 BBa_B0034 BBa_K801070 BBa_B0015 |