BBa_K113007

BBa_K113007 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K113007

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Wu Jingjing,Wang Jinyu

Date created: 2008-10-25 11:00:00

Date modified: 2015-05-08 01:09:20

pBad/araC->T7 polymerase

Details

• Types: DnaRegion
• Roles: engineered_region; Generator
• Sequences: BBa_K113007_sequence (Version 1)

Description

pBad is a promoter that is tightly controlled by an inducer (L-arabinose) and a repressor (araC). We use it to tightly control the expression of T7 polymerase as in our project the amount of T7 polymerase is essential.

Notes

We used standard assembly to combine pBad/araC and T7 polymerase. The restriction enzymes we used are SpeI and XbaI.

Source

BBa_I0500, iHKU team,

BBa_I712069

Components

Access	Instance	Definition
public	BBa_I0500	pBad/araC
public	BBa_I712069	BBa_I712069

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_I0500	1,1210	pBad/araC
BBa_I712069	1219,3896	BBa_I712069

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K113007/1

Member of these Collections

• iGEM Parts Registry